علوم زیستی دریا
Narges Saremi; Saeed Keyvanshokooh; Seyed Mohammad Mousavi; Hamid Mohammadiazarm
Abstract
Abstract
This study was conducted to investigate the effects of nano-selenium (Se) and vitamin C on the growth and nutritional indicators and body composition of common carp (Cyprinus carpio) juveniles. Experimental diets were prepared with three levels of nanoselenium (0, 0.5, and 1 mg/kg) and three ...
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Abstract
This study was conducted to investigate the effects of nano-selenium (Se) and vitamin C on the growth and nutritional indicators and body composition of common carp (Cyprinus carpio) juveniles. Experimental diets were prepared with three levels of nanoselenium (0, 0.5, and 1 mg/kg) and three levels of vitamin C (0, 500 and 1000 mg/kg). The fish were randomly divided into nine experimental groups in triplicate and fed with experimental diets for eight weeks. The results showed that fish fed with C500Se1 and C500Se0.5 diets had a significantly higher specific growth rate than other treatments (P<0.05). The lowest feed conversion ratio was observed in the C1000Se1 treatment and the highest in the control group (P<0.05). Also, based on the results, no significant change was observed in the carcass biochemical composition of common carp juveniles (P>0.05). In general, the results show that the composition of nanoselenium and vitamin C had positive effects on the growth and nutritional indicators of common carp, and it can be stated that the diet enriched with 500 mg of vitamin C and 1 mg of nanoselenium is recommended to increase the growth of common carp.
Introduction
Selenium (Se) is an essential micronutrient that participates in the structure of selenoproteins with vital biological functions in humans and animals. Selenium is involved in antioxidant defense, growth, fertility, immunity, digestion, and absorption of food in farmed animals, and has been used in nutritional studies on various fish species. Combined supplementation of nanoselenium and vitamin C improved growth performance and health status of Nile tilapia (Oreochromis niloticus) (Dawood et al., 2020). However, the positive effects of combining nanoselenium with vitamin C in common carp have not been investigated. As studies on other species suggest that the positive effects of nanoselenium can be enhanced in combination with other micronutrients (Khalil et al., 2022), the present study aimed to investigate the effects of nanoselenium and vitamin C on growth performance and body biochemistry of common carp (Cyprinus carpio) juveniles.
Materials and methods
Experimental diets were prepared with three levels of nanoselenium (0, 0.5 and 1 mg/kg) and three levels of vitamin C (0, 500 and 1000 mg/kg). The fish were randomly divided into 9 experimental groups in triplicates and were fed with experimental diets for 8 weeks. The fish were fed twice a day (9:00 am and 17:00 pm). Each experimental treatment was aerated with constant aeration and about 20% of water was daily exchanged. During the experiment, water quality parameters were daily monitored. Temperature ranges from 27.5 to 28.3oC, pH was between 7.5 and 7.8, dissolved oxygen was 7.2 to 7.5 mg/ml and photoperiods were about 10 hours of light and 14 hours of dark and was adjusted according to the daily natural light. At the end of the experimental period, all fish in each treatment were biometrically measured and the growth parameters were calculated.
Results
The results showed that fish fed with C500Se1 and C500Se0.5 diets had a significantly higher specific growth rate than other treatments (P<0.05). The lowest feed conversion ratio was observed in the C1000Se1 treatment and the highest in the control group (P<0.05). Also, based on the results, no significant change was observed in the carcass biochemical composition of common carp juvenile (P>0.05).
Conclusion
In the present study, the addition of nanoselenium and vitamin C, alone or in combination, to the diet of common carp increased the final weight and SGR and improved the FCR. These findings are in agreement with studies that investigated the effects of Nanoselenium or vitamin C supplements in their separate application on common carp (Ashouri et al., 2015; Ghafarifarsani et al., 2022) and other fish species (Çiçek and Özoğul, 2021, Dawood et al. 2021). In general, the results show that the composition of nanoselenium and vitamin C had positive effects on the growth and nutritional indicators of common carp and it can be stated that the diet enriched with 500 mg of vitamin C and 1 mg of nanoselenium is recommended to increase the growth of common carp.
علوم زیستی دریا
Iman Amirzadekani; Nasim Zanguee; Seyed Mohammad Mousavi; Mohammad Zakeri
Abstract
270 fish (with an average initial weight: 20.04±0.19 g) were randomly distributed in 27 polyethylene tanks and fed by the carp commercial diet. The experimental treatments of 9 groups (4 groups with 3, 6, 9, and 12% of alfalfa powder, 4 groups with 1, 2, 3, and 4% of alcoholic extract and one ...
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270 fish (with an average initial weight: 20.04±0.19 g) were randomly distributed in 27 polyethylene tanks and fed by the carp commercial diet. The experimental treatments of 9 groups (4 groups with 3, 6, 9, and 12% of alfalfa powder, 4 groups with 1, 2, 3, and 4% of alcoholic extract and one control group) with three replications were considered for 8 weeks. At the end of the experimental period, the activity of digestive enzymes was measured in terms of U/mg Protein and liver composition. Based on the results, the highest levels of activity of digestive enzymes, amylase (674.74±61.57), lipase (6/21±/096), alkaline phosphatase (452.84±28.18), trypsin (282.05±19.98) and chymotrypsin (0.10±0.001) in the extracts of 3%, 3%, 3%, 4% and 4% extracts, respectively, and the lowest amount of these enzymes were observed respectively with values of 388.17±38.27 (control), 2.51±0.55 (control), 187.7±19.36 (12% powder), 0.030±0.004 (12% powder) and 132.47±11.47 (9% powder) (P <0.05). The highest amount of hepatic glycogen (338.35±192.13 µg/g tissue) was measured in the 3% extract treatment and the lowest amount of hepatic glycogen (607.01±101.23 µg/g tissue was in the control treatment (P <0.05). The highest level of liver lipids (146.51±6.05 mg/g tissue) was observed in the treatment of 6% powder and the lowest level of liver lipids (72.99±4.46 mg/g tissue) was observed in the treatment of 12% powder (P <0.05). According to the present study, adding powder and especially alfalfa extract to the diet can increase the activity of digestive enzymes and increase liver lipid and glycogen at certain levels.
علوم زیستی دریا
Adel Ghabtani; Seyed Mohammad Mousavi; Annahita Rezaie; Mohammad Zakeri; Isac Zamani
Abstract
The purpose of this study was to evaluate the effects of three commercial probiotics (Bio-Aqua®, Bio-Aqua pond®, and Multi Behsil 100) on the growth, nutrition, and biochemical parameters of common carp fingerlings. For this purpose, 210 common carp fingerlings (with a mean weight: 9.6±0.02 ...
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The purpose of this study was to evaluate the effects of three commercial probiotics (Bio-Aqua®, Bio-Aqua pond®, and Multi Behsil 100) on the growth, nutrition, and biochemical parameters of common carp fingerlings. For this purpose, 210 common carp fingerlings (with a mean weight: 9.6±0.02 g and mean length: 12.9±0.32 cm) were randomly divided into 7 treatments (treatment 1 (control): basic diet without probiotics, treatment 2: 0.2 g of Bio-Aqua®, treatment 3: 0.1 g of Bio-Aqua®, treatment 4: 0.002 g/L of Bio-Aqua pond® dissolved in tank water, treatment 5: 0.001 g/L Bio-Aqua pond ® dissolved in tank water, treatment 6: 0.2 g of Multi Behsil 100, and treatment 7: 0.1 g of probiotic Multi Behsil 100) in triplicates, and 10 fish in each replicate. The commercial probiotics were added to the commercial diet, and the fish were fed based on body weight percentage for 60 days. Based on the results, the use of probiotics Bio-Aqua® and MultiBehsil 100, caused positive effects on the growth and nutrition parameters of common carp, although the use of 0.1 g of Bio-Aqua® per kilogram of the diet of common carp led to an improvement in growth, although, increasing the amount of this probiotic at the level of 0.2 g/kg led to negative effects on growth performance. The use of Multibehsil 100, caused positive effects on common carp. Thus, the best effect on growth performance and body composition was seen in the diet containing MultiBehsil 100 (0.1 and 0.2 g/kg of basic diet). Therefore, it can be concluded that the use of MultiBehsil 100 and Bio-Aqua® have favorable effects on common carp.
علوم زیستی دریا
Amin Ghaedtaheri; Saeed Keyvanshokouh; Seyed Mohammad Mousavi; Hossein Pasha-Zanoosi
Abstract
his study aimed to investigate the effect of selenium and magnesium nanoparticles and their composition on growth performance, nutritional indices, survival rate and hepatic accumulation of nano selenium and nano-magnesium in Asian Sea Bass (Lates calcalifer) with mean weight: 32.78 ± 1.16 g for ...
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his study aimed to investigate the effect of selenium and magnesium nanoparticles and their composition on growth performance, nutritional indices, survival rate and hepatic accumulation of nano selenium and nano-magnesium in Asian Sea Bass (Lates calcalifer) with mean weight: 32.78 ± 1.16 g for 42 days. After four weeks of acclimation to the experimental conditions, 96 fish were randomly distributed in 12 fiberglass 300-liter tanks in equal numbers. Four treatments including nano-selenium and nano-magnesium free as control group, 4 mg nano-selenium treatment, 0.5 g nano-magnesium treatment and combination of 4 mg nano-selenium and 0.5 g nano-magnesium were investigated in this study. The fish were fed daily in two times based on station. Fish biometry was performed in all treatments at the end of the experiment. Samples needed to measure liver accumulation were collected at the end of the period. The results of this study showed that the final weight in the treatment of selenium nanoparticles was significantly different (P <0.05), percentage of body weight gain and specific growth rate in selenium + magnesium nanoparticles treatment was significantly different compared to the control group (P <0.05). Nutritional indices of protein yield coefficient, feed intake and feed conversion ratio were not significantly different among experimental treatments (P> 0.05). The results showed that the survival rate was not significantly different among experimental treatments (P> 0.05). The highest levels of selenium and magnesium accumulation in liver were related to selenium nanoparticles and selenium + magnesium nanoparticles, respectively, which were significantly different from other treatments (P <0.05).
علوم زیستی دریا
Hamed Deilamy pour; Seyed Mohammad Mousavi; Mohammad Zakeri; Preeta Kochanian; Saeed Keyvanshokouh
Abstract
The aim of this study was to investigate the effect of selenium, magnesium nanoparticles and their composition on growth and digestive enzymes of Asian seabass with a mean weight of 32.78±1.16 g for 42 days. After adaptation to the experimental conditions, 96 fish were randomly distributed in ...
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The aim of this study was to investigate the effect of selenium, magnesium nanoparticles and their composition on growth and digestive enzymes of Asian seabass with a mean weight of 32.78±1.16 g for 42 days. After adaptation to the experimental conditions, 96 fish were randomly distributed in 12 fiberglass cylinder 300-liter tanks in equal numbers. Four treatments including control treatment, 4mg / kg nanoselenium, 500mg / kg nanomagnasium and combination of 4mg/ kg nanoselenium with 500mg / kg nanomagnasium were investigated. The fish were fed daily in satiation and up to 3% of body weight in two time a day. Samples needed to measure growth indices and digestive enzymes were collected at the end of the period. The results showed that in the fish fed the combined treatment, the highest body weight gain was 131.33±26.58 and the specific growth rate was 2.08±0.28 and there was a significant difference between the control treatment (65.01±21.26 and 1.23±0.32, respectively) (P <0.05). The results of this study showed that digestive enzymes of alkaline phosphatase, lipase, trypsin and chymotrypsin were significantly different between experimental treatments (P <0.05), so that the highest activity of alkaline phosphatase in nanoselenium treatment (611.06±82.00U/mg protein), the highest amount of lipase (0.86±0.03 U/mg protein), trypsin (0.054 ± 0.001 U/mg protein), and chymotrypsin (0.214±0.004U/mg protein) was seen in nanomagnesium treatment (P <0.05), whereas the amylase enzyme showed no significant difference between the experimental treatments (P> 0.05). The results of this study showed that the addition of selenium and magnesium nanoparticles to the diet of Asian Sea bass had positive effects on growth performance and digestive enzymes and suggested that magnesium nanoparticle supplementation (500 mg/kg) could be used alone or in combination with selenium nanoparticles (4 mg/kg nano-selenium and 500 mg / kg nano-magnesium) in the diet of Asian Sea bass.
علوم زیستی دریا
Simin Ghoreishvandi; s m; Seyyed Nasrollah Hosseini; Annahita Rezaie
Abstract
In this study, post-mortem changes, including chemical, physical, microbial and sensorial changes, were performed in “Gatan” during ice storage. For this purpose, 18 fishes with Average weight 251.4 ± 4.24 g were transferred to the wet laboratory of Khorramshahr University of Marine ...
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In this study, post-mortem changes, including chemical, physical, microbial and sensorial changes, were performed in “Gatan” during ice storage. For this purpose, 18 fishes with Average weight 251.4 ± 4.24 g were transferred to the wet laboratory of Khorramshahr University of Marine Science and Technology, and in order to adapt to the laboratory conditions and to remove the stress caused by displacement, They were kept under favorable conditions for a week. Samples were catch immediately stored in ice for 72 hours, and meat quality indices were measured at 0, 6, 12, 24, 48 and 72 hours after death. The results showed that pH, TBA, FFA indices increased during storage period, TVBN initially decreased and then increased Microbiological studies also showed that the Cyrophile bacteria had no growth during the maintenance period. The amount of mesophilic bacteria at time zero was 2.53 ± 1.53 and then at 6, 12, and 24, it was descending process towards the zero hour. It then gradually increased and maintained at 1.72 ± 0.72 at the Seventy second hour of storage. Sensory evaluation of the fish showed that they had a suitable and acceptable condition for up to 24 hours after storage at the ice. During the maintenance period, the gradual decrease in acceptability was reduced and at 72 hours, most of the sensory analysis indexes obtained lower scores, and samples at that hour were the lowest quality during the maintenance period. Therefore, the use of ice to maintain long-term fish is not an appropriate method.
علوم زیستی دریا
zahra masoomi; mohammad zakeri; seied mohammad mousavi; vahid yavari
Abstract
In this study, the effects of different dietary protein and salinity levels on cellular parameters of hemolymph in white leg shrimp (as a part of immune system) of the white leg shrimp (Litopenaeus vannamei) juvenile were studied. The number of 350 shrimps with the average weight of 5.55±0.18 ...
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In this study, the effects of different dietary protein and salinity levels on cellular parameters of hemolymph in white leg shrimp (as a part of immune system) of the white leg shrimp (Litopenaeus vannamei) juvenile were studied. The number of 350 shrimps with the average weight of 5.55±0.18 g and length of 8.81±0.15 cm were distributed randomly in 27 ten tones (length: 600 cm, width: 170 cm and height: 100 cm) concrete tanks. A 3×3 factorial experimental including three levels of salinity (0-3, 12-15 and 32-35 ppt) and three dietary protein levels (25, 35 and 45%). Experimental shrimps were fed to satiation 4 times a day throughout the experimental 56 days. According to the results cellular parameters like: total hemocytes count (THC) and differential hemocyte count (DHC) were analysed and recorded. The dietary protein levels 35% and salinity of 32-35 ppt had direct positive impact on THC and granular cells (GC). Although the semi granular poisonous cells have been decreased with the increase in both of the experimental variables (P≤ 0/05) and no effected on the agranular cells. So based on the results of this study, the protein diet with the 35% is suggested for L. vannamei. Taking in to consideration the results of the study we can say the best water salinity for culture of this species is 32-35 ppt.
علوم زیستی دریا
mohammad rahimi; s m; Vahid Yavari; Annahita Rezaie
Abstract
For study of changes in energy metabolism of brain in rainbow trout during early life stages, some samples were collected from fish larvae and fingerlings with initial weight 1.35±0.15 during 7 months. Sample collection was randomly made in 12 stages (7 samples per every 8 days, 3 samples per ...
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For study of changes in energy metabolism of brain in rainbow trout during early life stages, some samples were collected from fish larvae and fingerlings with initial weight 1.35±0.15 during 7 months. Sample collection was randomly made in 12 stages (7 samples per every 8 days, 3 samples per every 2 weeks and 2 samples every one month). Brain tissue was dissected and transferred to nitrogen tank before analysis. The brain tissues were homogenized and the supernatant was adjusted to measurement of glycogen, glucose, lactate, lactate dehydrogenase, pyruvate kinase and glucose-6- phosphatase. Results revealed statistically changes in the metabolites during the sampling, so, glycogen and lactate concentration showed an increasing trend by increasing in fish age, but glucose concentration did not follow this trend. The minimum glucose concentration in brain tissue was observed in seventh stage of sampling. Concentration of metabolism enzymes was showed fluctuations and increasing trend during sampling. Finally, it is proposed that environmental and nutritional factors and different requirement of fish to energy in different stages of life may be effective on metabolites concentrations and metabolism enzymes activity.
علوم زیستی دریا
Asad Asadi Eidivand; Seyed Mohammad Mousavi; Seyed Reza Fatemi Tabatabaei; Mohammad Zakeri; Nasim Zanguee
Abstract
This research was conducted for studying of biological parameters of Tenualosa ilisha on spawning and post spawning time. For this reason, 60 fish were captured through three months, on September- fresh water (Karoon river in Khorramshahr) (1st station), September- marine water (Hendijan coasts) (2nd ...
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This research was conducted for studying of biological parameters of Tenualosa ilisha on spawning and post spawning time. For this reason, 60 fish were captured through three months, on September- fresh water (Karoon river in Khorramshahr) (1st station), September- marine water (Hendijan coasts) (2nd station), October-marine water (3rd station) and November-marine water (4th station). Biological parameters, GSI and gonad histological study was performed. The results showed the highest total length, total weight and gonad weight were seen in fish were captured from 1st station which has a significant different with other fishes (p
علوم زیستی دریا
Ali Arshadi; Vahid Yavari; Amin Oujifard; Seyed Mohammad Mousavi
Abstract
In this study, the effect of different dietary nucleotide levels (0, 0.2, 0.4 and 0.6%) was investigated on growth indices and some haemolymph biochemical parameters of Litopenaeus vannamei female broodstock during 30 days.hemolymph was sampled in three times, at the first day of the trial, 21 days (before ...
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In this study, the effect of different dietary nucleotide levels (0, 0.2, 0.4 and 0.6%) was investigated on growth indices and some haemolymph biochemical parameters of Litopenaeus vannamei female broodstock during 30 days.hemolymph was sampled in three times, at the first day of the trial, 21 days (before eyestalk ablation) and 30 days (9 days after eyestalk ablation) after starting the feeding trial. In experimental period, water parameters including temperature, dissolve oxygen, salinity and pH recorded daily as 27.79 ± 1.24 ˚C, 5.78 ± 0.41 ppm, 31.82 ± 3.1 ppt and 7.9 ± 0.18, respectively. haemolymph biochemical parameters of female broodstock including glucose, total cholesterol, triglyceride, calcium, albumin, total soluble proteins, high density lipoprotein and low density lipoprotein measured in three phases (start of feeding, 21 [pre eyestalk ablation period] and 30 days after start feeding) by biochemical standard methods. the results showed that body weight increase, specific growth rate and daily growth rate in samples treated by dietary nucleotide showed no significant difference (p > 0.05) compared to control group. analysis of biochemical heamolymph showed that the glucose, total cholesterol, triglyceride, albumin, total protein were significant increasing (p < 0.05), in all groups compare to control group. In conclusion 0.4 % nucleotide can improve the dietary sexual maturation of Litopenaeus vannamei.
علوم زیستی دریا
TAYEBEH ASADI; n z; s m; v y
Abstract
In the recent decades, using of herbs and herbal extracts as growth promoters and immunostimulants in aquaculture has been increased. In the current study, effects of Ginger (Zingiber officinale) extract on hematological and some serological parameters and growth performance of Barbus sharpeyi (Gunther, ...
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In the recent decades, using of herbs and herbal extracts as growth promoters and immunostimulants in aquaculture has been increased. In the current study, effects of Ginger (Zingiber officinale) extract on hematological and some serological parameters and growth performance of Barbus sharpeyi (Gunther, 1874( fingerlings were studied. Four levels of ginger extract including 0 (Control group), 0.1, 0.5 and 1 per cent were added to basic diet. Fish were fed twice a day as 3 per cent of body weight for 8 weeks. At the end of the experimental period, biometry was performed and blood samples were collected from caudal vein. The results have shown that administration of ginger extract supplemented diets did not significantly affect growth indexes (WG and SGR) (p ≥ 0.05). On the other hand, different levels of ginger extract could significantly increase WBC count, hematocrit and some hematological parameters. Also, Serum lysozyme activity and serum total protein and albumin were significantly increased after using ginger extract (p ≤ 0.05). based on the results obtained from this study, ginger extract may be used as a growth promoter and immunostimulant in Barbus sharpeyi.